136545 wtmbp Search Results


136545 wtmbp  (Addgene inc)
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Addgene inc 136545 wtmbp
Figure 4. Crystal Screening of Different <t>wtMBP</t> and ANXA2 Constructs (A and B) (A) ANXA229339 and (B) PDZ-ANXA223339 crystals were formed under numerous JCSG+ (blue) and Morpheus (red) crystallization conditions at 250 mM concentration. (C–F) (C) In the case of the larger scS100A4D8-ANXA229339 construct, crystal formation significantly dropped. Crystal formation of (D) wtMBP, (E) wtMBP-PDZ, and (F) wtMBP-scS100A4D8 at 2 mM concentration is also presented. Crystal growth was monitored for a month.
136545 Wtmbp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. Crystal Screening of Different wtMBP and ANXA2 Constructs (A and B) (A) ANXA229339 and (B) PDZ-ANXA223339 crystals were formed under numerous JCSG+ (blue) and Morpheus (red) crystallization conditions at 250 mM concentration. (C–F) (C) In the case of the larger scS100A4D8-ANXA229339 construct, crystal formation significantly dropped. Crystal formation of (D) wtMBP, (E) wtMBP-PDZ, and (F) wtMBP-scS100A4D8 at 2 mM concentration is also presented. Crystal growth was monitored for a month.

Journal: Structure (London, England : 1993)

Article Title: Structure Determination of the Transactivation Domain of p53 in Complex with S100A4 Using Annexin A2 as a Crystallization Chaperone.

doi: 10.1016/j.str.2020.05.001

Figure Lengend Snippet: Figure 4. Crystal Screening of Different wtMBP and ANXA2 Constructs (A and B) (A) ANXA229339 and (B) PDZ-ANXA223339 crystals were formed under numerous JCSG+ (blue) and Morpheus (red) crystallization conditions at 250 mM concentration. (C–F) (C) In the case of the larger scS100A4D8-ANXA229339 construct, crystal formation significantly dropped. Crystal formation of (D) wtMBP, (E) wtMBP-PDZ, and (F) wtMBP-scS100A4D8 at 2 mM concentration is also presented. Crystal growth was monitored for a month.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA ANXA223-339 in modified pET15b vector (pANXA223-339) This paper Addgene ID: 136543 and 136546 ANXA2 29-339 in modified pET15b vector (pANXA229-339) This paper Addgene ID: 136544 and 136545 wtMBP in in pET-MBP This paper N/A P53 TAD17-56 – scS100A4D8 – ANXA229-339 in modified pET15b vector (pANXA229-339) This paper N/A scS100A4D8 – ANXA229-339 in modified pET15b vector (pANXA229-339) This paper N/A PDZ – ANXA223-339 in modified pET15b vector (pANXA223-339) Gogl et al., 2018 N/A wtMBP – scS100A4D8 in pET-MBP This paper N/A wtMPB – PDZ in pET-MBP Gogl et al., 2018 N/A PDZ in pET-MBP Gogl et al., 2018 N/A scS100A4D8 in modified pET15b vector (pEV) This paper N/A S100A4 in modified pET15b vector (pEV) Kiss et al., 2012 N/A P53 TAD1-60 in modified pGEX vector (pETARA) This paper N/A P53 TAD17-56 peptide in modified pGEX vector (pETARA) This paper N/A P53 TAD17-56 peptide mutants in modified pGEX vector (pETARA) This paper N/A Tobacco etch virus (TEV) protease in pTH24 vector van den Berg et al., 2006 N/A Software and Algorithms Coot Emsley et al., 2010 https://www2.mrc-lmb.cam.ac.uk/personal/ Phenix Adams et al., 2010 https://www.phenix-online.org Pymol PYMOL http://www.pymol.org Phaser McCoy, 2007 http://www-structmed.cimr.cam.ac.uk/ phaser_obsolete/ XDS Kabsch, 2010 http://xds.mpimf-heidelberg.mpg.de/ Origin for ITC 5.0 OriginLab http://www.originlab.com/ Origin 8 OriginLab http://www.originlab.com/ GeneTools GeneTools http://www.syngene.com/genetools-software- download Bestsel Micsonai et al., 2015 http://bestsel.elte.hu/ Other Phenyl Sepharose 6 Fast Flow GE Healthcare #17-0973-05 HiTrap SP HP cation exchange column GE Healthcare #17115101 Jupiter 300 C5 column Phenomenex #00G-4052-E0 384-well microplates Corning #3676 Profinity IMAC resin Bio-Rad #1560131 Protino Glutathione Agarose 4B resin Macherey-Nagel #745500.10 Amylose resin NEB #E8021S HighTrap Heparin HP column GE Healthcare (Sigma) #GE17-0406-01 HiTrap Desalting column GE Healthcare (Sigma) #GE29-0486-84

Techniques: Construct, Crystallization Assay, Concentration Assay

Figure 5. Crystallization of ANXA2 Constructs and wtMBP at Different Concentrations Using the Best Conditions Found in the Screening Experiments Formation of (A) ANXA229339 (black), PDZ-ANXA223339 (gray), and scS100A4D8-ANXA229339 (horizontal lines) crystals were monitored at different protein concentrations for a month. (B) MBP crystals (vertical lines) formed only at higher than 500 mM concentration (~20.5 mg/mL). No MBP fused constructs were tested since no hit was found during screening. (C–E) (C) PDZ-ANXA223339, (D) ANXA229339, and (E) wtMBP crystals when samples were used at 250 mM (2 mM), 62.5 mM (1 mM), and 7 mM concentrations (0.5 mM) from left to right (wtMBP concentrations are in parenthesis). Small crystals are highlighted in white boxes. White bars represent 0.5 mm.

Journal: Structure (London, England : 1993)

Article Title: Structure Determination of the Transactivation Domain of p53 in Complex with S100A4 Using Annexin A2 as a Crystallization Chaperone.

doi: 10.1016/j.str.2020.05.001

Figure Lengend Snippet: Figure 5. Crystallization of ANXA2 Constructs and wtMBP at Different Concentrations Using the Best Conditions Found in the Screening Experiments Formation of (A) ANXA229339 (black), PDZ-ANXA223339 (gray), and scS100A4D8-ANXA229339 (horizontal lines) crystals were monitored at different protein concentrations for a month. (B) MBP crystals (vertical lines) formed only at higher than 500 mM concentration (~20.5 mg/mL). No MBP fused constructs were tested since no hit was found during screening. (C–E) (C) PDZ-ANXA223339, (D) ANXA229339, and (E) wtMBP crystals when samples were used at 250 mM (2 mM), 62.5 mM (1 mM), and 7 mM concentrations (0.5 mM) from left to right (wtMBP concentrations are in parenthesis). Small crystals are highlighted in white boxes. White bars represent 0.5 mm.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA ANXA223-339 in modified pET15b vector (pANXA223-339) This paper Addgene ID: 136543 and 136546 ANXA2 29-339 in modified pET15b vector (pANXA229-339) This paper Addgene ID: 136544 and 136545 wtMBP in in pET-MBP This paper N/A P53 TAD17-56 – scS100A4D8 – ANXA229-339 in modified pET15b vector (pANXA229-339) This paper N/A scS100A4D8 – ANXA229-339 in modified pET15b vector (pANXA229-339) This paper N/A PDZ – ANXA223-339 in modified pET15b vector (pANXA223-339) Gogl et al., 2018 N/A wtMBP – scS100A4D8 in pET-MBP This paper N/A wtMPB – PDZ in pET-MBP Gogl et al., 2018 N/A PDZ in pET-MBP Gogl et al., 2018 N/A scS100A4D8 in modified pET15b vector (pEV) This paper N/A S100A4 in modified pET15b vector (pEV) Kiss et al., 2012 N/A P53 TAD1-60 in modified pGEX vector (pETARA) This paper N/A P53 TAD17-56 peptide in modified pGEX vector (pETARA) This paper N/A P53 TAD17-56 peptide mutants in modified pGEX vector (pETARA) This paper N/A Tobacco etch virus (TEV) protease in pTH24 vector van den Berg et al., 2006 N/A Software and Algorithms Coot Emsley et al., 2010 https://www2.mrc-lmb.cam.ac.uk/personal/ Phenix Adams et al., 2010 https://www.phenix-online.org Pymol PYMOL http://www.pymol.org Phaser McCoy, 2007 http://www-structmed.cimr.cam.ac.uk/ phaser_obsolete/ XDS Kabsch, 2010 http://xds.mpimf-heidelberg.mpg.de/ Origin for ITC 5.0 OriginLab http://www.originlab.com/ Origin 8 OriginLab http://www.originlab.com/ GeneTools GeneTools http://www.syngene.com/genetools-software- download Bestsel Micsonai et al., 2015 http://bestsel.elte.hu/ Other Phenyl Sepharose 6 Fast Flow GE Healthcare #17-0973-05 HiTrap SP HP cation exchange column GE Healthcare #17115101 Jupiter 300 C5 column Phenomenex #00G-4052-E0 384-well microplates Corning #3676 Profinity IMAC resin Bio-Rad #1560131 Protino Glutathione Agarose 4B resin Macherey-Nagel #745500.10 Amylose resin NEB #E8021S HighTrap Heparin HP column GE Healthcare (Sigma) #GE17-0406-01 HiTrap Desalting column GE Healthcare (Sigma) #GE29-0486-84

Techniques: Crystallization Assay, Construct, Concentration Assay